Analisis Makna Pada Kring Solopos Edisi Bulan November 2014:Tinjauan Semantik

Makna merupakan suatu wujud simbol yang digunakan seseorang dalam melakukan suatu perbuatan. Tanda dapat berupa sebuah gerakan anggota badan, bunyi ujaran atau bunyi bahasa dan sebagainya. Demikian pula suatu pesan yang ditulis oleh manusia mempunyai makna dan tujuan dibalik pembuatannya. Adapun jenis makna yang dikaji dalam penelitian ini yaitu makna denotatif atau kognitif dan makna konotatif atau emotif. Tujuan dari penelitian yaitu (1) mendeskripsikan makna konotatif yang terkandung dalam Kring Solopos edisi bulan November 2014, (2) mendiskripsikan makna denotatif yang terkandung dari Kring Solopos edisi bulan November 2014 (3) mendiskripsikan makna berdasarkan tinjauan semantik. Metode penelitian menggunakan metode deskriptif kualitatif. Penelitian ini menggunakan strategi penelitian ini adalah analisis isi. Teknik pengumpulan data yang dilakukan dalam penelitian ini menggunakan metode simak dengan teknik lanjutan yaitu teknik catat. Teknik keabsahan data menggunakan trianggulasi teori. Metode yang digunakan untuk menganalisis data adalah metode padan dan metode agih. Hasil analisis dalam penelitian ini berupa kalimat yang mengandung makna denotatif atau kognitif dan konotatif atau emotif. Simpulan pada penelitian yaitu bahwa dari 40 pesan yang diambil terdapat 105 kalimat yang mengandung sebuah makna leksikal terdiri atas makna denotatif dan konotatif. Kalimat yang mengandung makna denotatif/kognitif lebih banyak dibandingkan kalimat yang mengandung makna konotatif/emotif

Affiliated Keyword Search Cognomiate Reviewer and Indite Accredit Envoy Inscription Province for E-Harch Clouds

We present a novel cryptographic primitive named as conjunctive keyword search with assigned analyzer and timing empowered intermediary re-encryption work (Re-dtPECK), which is a sort of a period subordinate SE conspire. It could empower patients to appoint incomplete access rights to others to work search works over their records in a constrained day and age. The length of the day and age for the delegatee to search and decode the delegator's scrambled reports can be controlled. Also, the delegatee could be naturally denied of the entrance and inquiry expert after a predetermined time of compelling time. It can likewise bolster the conjunctive keywords hunt and oppose the keyword speculating assaults. By the arrangement, just the assigned analyzer can test the presence of specific keywords. We define a framework demonstrate and a security display for the proposed Re-dtPECK plan to demonstrate that it is an effective plan demonstrated secure in the standard model

Analisa Pengaruh Authentic Leadership Terhadap Employee Engagement Di Hotel “X” Surabaya

Penelitian ini bertujuan untuk mengetahui pengaruh authentic leadership terhadap employee engagement di Hotel “X” Surabaya. Penelitian ini melibatkan 138 karyawan Hotel “X” Surabaya. Jenis penelitian yang digunakan adalah kuantitatif kausal. Analisa pengaruh menggunakan analisa regresi linear berganda. Hasil penelitian menunjukkan bahwa authentic leadership berpengaruh simultan dan signifikan namun dari empat sub variabel authentic leadership, tiga diantaranya berpengaruh secara parsial namun tidak signifikan. Hanya satu sub variabel saja yang memiliki pengaruh secara parsial dan signifikan terhadap employee engagement. Sub variabel internalized moral persective adalah sub variabel paling dominan terhadap employee engagement di hotel “X” Surabaya

Pengaruh Brain Gym Terhadap Penurunan Tingkat Stres Pada Remaja Kelas VII SMP Muhammadiyah Pekalongan

Latar belakang : Setiap manusia pasti pernah mengalami stres, Perubahan fisik pada diri remaja dalam masa pubertas terkadang menyebabkan ketidakstabilan emosi dan berpotensi terjadinya stres. Stres pada remaja awal lebih di akibatkan oleh faktor usia transisi dari anak-anak menuju dewasa periode ini disebut periode storm and stres. Fisioterapi dapat mengurangi terjadinya stres pada masa awal pubertas dengan memberikan Brain Gym, sebagai salah satu upaya untuk mengurangi derajat stres pada remaja agar mereka dapat berpotensi secara optimal. Tujuan Penelitian : Mengetahui pengaruh latihan Brain Gym terhadap penurunan tingkat stres pada remaja kelas VII SMP Muhammadiyah Pekalongan dan untuk mengetahui perbedaan pengaruh latihan Brain Gym terhadap penurunan tingkat stres pada remaja kelas VII SMP Muhammadiyah Pekalongan Metode penelitian : Menggunakan metode Quasi Experiment dengan pre and post test without control group design. Jumlah responden yang di libatkan ada 36 responden. Derajat stres diukur dengan skala DASS (Depression Anxiety and Stress Scale). Pemberian Brain Gym dilakukan secara rutin 12 kali selama 2 minggu dengan durasi 10 – 15 menit. Hasil dan kesimpulan: Ada pengaruh pemberian Brain Gym terhadap penurunan tingkat stres pada remaja kelas VII SMP Muhammadiyah Pekalongan Hal ini dapat dilihat dari hasil uji beda pengaruh bahwa pada saat ditest menggunakan skala DASS setelah mendapatkan perlakuan Brain Gym diperoleh p-value sebesar 0.000 karena p-value lebih kecil dari 0,05 kesimpulan Signifikan

Pengaruh Stres Kerja, Lingkungan Kerja, Dan Komitmen Organisasi Terhadap Kinerja Pegawai Pada Satuan Polisi Pamong Praja Kabupaten Karanganyar

The aim to be achieved by holding this research is to determine the effect of work stress, work environment, and organizational commitment on employee performance. This research was conducted in the Civil Service Police Unit of Karanganyar Regency. The type of research used is quantitative research, the data used in this study is primary data through questionnaires. The sample used in this study was 101 respondents using the proportional cluster random sampling technique. The data collection method used is a questionnaire. The data analysis technique used is validity test, reliability test, classical assumption test, and multiple linear regression analysis. The results of this study indicate that the work stress variable has a negative and significant effect on the employee performance variable while the work environment variable and organizational commitment have no significant effect on employee performance. employee performance variable in the Unit. Karanganyar Regency Civil Service Police

Regulation of immune responses in primary biliary cholangitis: a transcriptomic analysis of peripheral immune cells

BACKGROUND AIMS: In patients with primary biliary cholangitis (PBC), the serum liver biochemistry measured during treatment with ursodeoxycholic acid-the UDCA response-accurately predicts long-term outcome. Molecular characterization of patients stratified by UDCA response can improve biological understanding of the high-risk disease, thereby helping to identify alternative approaches to disease-modifying therapy. In this study, we sought to characterize the immunobiology of the UDCA response using transcriptional profiling of peripheral blood mononuclear cell subsets. METHODS: We performed bulk RNA-sequencing of monocytes and TH1, TH17, TREG, and B cells isolated from the peripheral blood of 15 PBC patients with adequate UDCA response ("responders"), 16 PBC patients with inadequate UDCA response ("nonresponders"), and 15 matched controls. We used the Weighted Gene Co-expression Network Analysis to identify networks of co-expressed genes ("modules") associated with response status and the most highly connected genes ("hub genes") within them. Finally, we performed a Multi-Omics Factor Analysis of the Weighted Gene Co-expression Network Analysis modules to identify the principal axes of biological variation ("latent factors") across all peripheral blood mononuclear cell subsets. RESULTS: Using the Weighted Gene Co-expression Network Analysis, we identified modules associated with response and/or disease status (q<0.05) in each peripheral blood mononuclear cell subset. Hub genes and functional annotations suggested that monocytes are proinflammatory in nonresponders, but antiinflammatory in responders; TH1 and TH17 cells are activated in all PBC cases but better regulated in responders; and TREG cells are activated-but also kept in check-in responders. Using the Multi-Omics Factor Analysis, we found that antiinflammatory activity in monocytes, regulation of TH1 cells, and activation of TREG cells are interrelated and more prominent in responders. CONCLUSIONS: We provide evidence that adaptive immune responses are better regulated in patients with PBC with adequate UDCA response

New Pathogenesis Mechanisms and Translational Leads Identified by Multidimensional Analysis of Necrotizing Myositis in Primates

A fundamental goal of contemporary biomedical research is to understand the molecular basis of disease pathogenesis and exploit this information to develop targeted and more-effective therapies. Necrotizing myositis caused by the bacterial pathogen Streptococcus pyogenes is a devastating human infection with a high mortality rate and few successful therapeutic options. We used dual transcrip-tome sequencing (RNA-seq) to analyze the transcriptomes of S. pyogenes and host skeletal muscle recovered contemporaneously from infected nonhuman primates. The in vivo bacterial transcriptome was strikingly remodeled compared to organisms grown in vitro, with significant upregulation of genes contributing to virulence and altered regulation of metabolic genes. The transcriptome of muscle tissue from infected nonhuman primates (NHPs) differed significantly from that of mock-infected animals, due in part to substantial changes in genes contributing to inflammation and host defense processes. We discovered significant positive correlations between group A streptococcus (GAS) virulence factor transcripts and genes involved in the host immune response and inflammation. We also discovered significant correlations between the magnitude of bacterial virulence gene expression in vivo and pathogen fitness, as assessed by previously conducted genome-wide transposon-directed insertion site sequencing (TraDIS). By integrating the bacterial RNA-seq data with the fitness data generated by TraDIS, we discovered five new pathogen genes, namely, S. pyogenes 0281 (Spy0281 [dahA]), ihk-irr, slr, isp, and ciaH, that contribute to necrotizing myositis and confirmed these findings using isogenic deletion-mutant strains. Taken together, our study results provide rich new information about the molecular events occurring in severe invasive infection of primate skeletal muscle that has extensive translational research implications. IMPORTANCE Necrotizing myositis caused by Streptococcus pyogenes has high morbidity and mortality rates and relatively few successful therapeutic options. In addition, there is no licensed human S. pyogenes vaccine. To gain enhanced understanding of the molecular basis of this infection, we employed a multidimensional analysis strategy that included dual RNA-seq and other data derived from experimental infection of nonhuman primates. The data were used to target five streptococcal genes for pathogenesis research, resulting in the unambiguous demonstration that these genes contribute to pathogen-host molecular interactions in necrotizing infections. We exploited fitness data derived from a recently conducted genome-wide transposon mutagenesis study to discover significant correlation between the magnitude of bacterial virulence gene expression in vivo and pathogen fitness. Collectively, our findings have significant implications for translational research, potentially including vaccine efforts.Peer reviewe

Population Genomic Molecular Epidemiological Study of Macrolide-Resistant Streptococcus pyogenes in Iceland, 1995 to 2016: Identification of a Large Clonal Population with a pbp2x Mutation Conferring Reduced In Vitro β-Lactam Susceptibility

Publisher's version (útgefin grein)Resistance to macrolide antibiotics is a global concern in the treatment of Streptococcus pyogenes (group A Streptococcus [GAS]) infections. In Iceland, since the detection of the first macrolide-resistant isolate in 1998, three epidemic waves of macrolide-resistant GAS infections have occurred, with peaks in 1999, 2004, and 2008. We conducted whole-genome sequencing of all 1,575 available GAS macrolide-resistant clinical isolates of all infection types collected at the national reference laboratory in Reykjavik, Iceland, from 1998 to 2016. Among 1,515 erythromycin-resistant isolates, 90.3% were of only three emm types, emm4 (n = 713), emm6 (n = 324), and emm12 (n = 332), with each being predominant in a distinct epidemic peak. The antibiotic efflux pump genes, mef(A) and msr(D), were present on chimeric mobile genetic elements in 99.3% of the macrolide-resistant isolates of these emm types. Of note, in addition to macrolide resistance, virtually all emm12 isolates had a single amino acid substitution in penicillin-binding protein PBP2X that conferred a 2-fold increased penicillin G and ampicillin MIC among the isolates tested. We conclude that each of the three large epidemic peaks of macrolide-resistant GAS infections occurring in Iceland since 1998 was caused by the emergence and clonal expansion of progenitor strains, with macrolide resistance being conferred predominantly by inducible Mef(A) and Msr(D) drug efflux pumps. The occurrence of emm12 strains with macrolide resistance and decreased beta-lactam susceptibility was unexpected and is of public health concern.This study was supported in part by the Fondren Foundation, Houston Methodist Hospital and Research Institute, and National Institutes of Health grants AI139369 and AI146771 (to J.M.M.).Peer Reviewe